Electroanalytical/X-ray photoelectron spectroscopy investigation on glucose oxidase adsorbed on platinum
- 1 January 1994
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Faraday Transactions
- Vol. 90 (11) , 1495-1499
- https://doi.org/10.1039/ft9949001495
Abstract
Glucose oxidase has been absorbed on Pt from a solution of the enzyme (0.5–15 µmol dm–3) in phosphate or acetate buffer (pH 3.9–8.0). The amperometric response to glucose of the modified electrodes so prepared has been evaluated. A spectroscopic (XPS) investigation of the system surface indicates that the enzyme molecules are stacked on the Pt surface, some directly interacting with the metal and the remaining part adsorbed on them. At the surface, the enzyme has a conformation in which its carbohydrate part tends to cover the proteic portion.Keywords
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