THE SEPARATION AND IDENTIFICATION OF MICROQUANTITIES OFRAUWOLFIAALKALOIDS

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Abstract
The techniques employed for establishment of the identities of the separated alkaloids are based on physical methods. For satisfactory identification and authentication, comparison with reference compounds is desirable. Ultraviolet spectrophotometric data provides a means of allocating the alkaloids to their respective chromophoric groups and absorbancy ratios offer a further guide to identity. Infrared spectral data yields information on the occurrence of characteristic, functional groups. Mass spectra provide additional facts concerning various fragments and substituent groups of the molecules as well as defining molecular weights and offering limited information concerning stereochemistry. The combination of spectrometric information with the results of Chromatographie separations, fluorescence and chromogenic tests and melting point determinations permits the identification of micro-quantities of alkaloids. Thus, in the roots of Rauwolfia caffra Sond., we have identified aricine (A 1 ), ajmalicine (A 2 ), reserpine (B 1 ), rescinnamine (B 2 ), renoxidine (PLC), yohimbine (C), raucaffricine (D), ajmaline (E), sarpagine (PLC) and serpentine (F) using a minimum of 60 g of root bark.

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