Evidence that the Antigen of Autologous Immune Complex Glomerulonephritis of Rats is a Mannose- or Glucose-Containing Glycoprotein

Publisher Website
Google Scholar
Abstract
Chemical nature of the brush border antigen (BBAg) of the autologous immune complex glomerulonephritis (AIC) of rats was investigated by a histochemical method utilizing indirect immunofluorescence. Fresh frozen rat kidney sections, a specific antibody to BBAg (BBAb), purified lectins (concanavalin[Con]A, Ricin II, wheat germ hemagglutinin [WGA]) and a specific sugar, .alpha.-methyl-D-mannopyranoside, were used. The reaction of the specific antibody BBAb to its antigen BBAg on tissue sections was blocked by ConA but not by Ricin II and WGA. This blockade was reversed by .alpha.-methyl-D-mannopyranoside. The antigen of AIC of rats is apparnetly a mannose or glucose containing glycoprotein. Identification of the chemical nature of the antigen of AIC is of considerable importance because AIC has a striking clinical, histological and immunohistological similarity to the idiopathic membranous glomerulonephritis in humans.

This publication has 0 references indexed in Scilit: