Elimination of the "chromogen oxidase" activity of bilirubin oxidase added to obviate bilirubin interference in hydrogen peroxide/peroxidase detecting systems.
Open Access
- 1 December 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 31 (12) , 2007-2008
- https://doi.org/10.1093/clinchem/31.12.2007
Abstract
The use of bilirubin oxidase to remove interference by bilirubin in hydrogen peroxide/peroxidase detecting systems is hampered by its inherent "chromogen oxidase" activity (its ability to oxidize the chromogens used in the systems). This unwanted activity is greater than 99% inhibited by 0.5 mmol/L cyanide, 97% inhibited by 20 mmol/L azide. At these same concentrations, they inhibit bilirubin oxidase activity by 95% and 73%, respectively. Sequential addition of reagents allows the use of bilirubin oxidase without interference by the chromogen oxidase activity.This publication has 0 references indexed in Scilit: