Axenic Cultivation ofPhytomonas davidiLafont (Trypanosomatidae), a Symbiote of Laticiferous Plants (Euphorbiaceae)*
- 1 May 1976
- journal article
- Published by Wiley in The Journal of Protozoology
- Vol. 23 (2) , 238-241
- https://doi.org/10.1111/j.1550-7408.1976.tb03761.x
Abstract
SYNOPSIS.Phytomonas davidi(Trypanosomatidae) originally discovered by Lafont in 1909 on the island of Mauritius was rediscovered inEuphorbia cyathophorain Florida. Successful cultures were established in diphasic medium consisting of duck blood agar and modified Phillips’medium as overlay. Optimal growth was obtained when Mansour's medium was used as overlay and poorest growth when Cowperthwaite's medium buffered at pH 5.0 was utilized for this purpose. Marked changes tending toward choanomastigotes rather than the elongate twisted promastigotes were observed in cultures.Keywords
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