Quantitative Paper Chromatography of Fatty Acids. I. The Influence of Residual Mobile Phase.
- 1 January 1963
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 17 (1) , 139-143
- https://doi.org/10.3891/acta.chem.scand.17-0139
Abstract
Use of test mixtures of C14 labelled acids and scanning of both radioactivity and color has enabled a detailed study of the correlation between photometric readings and the corresponding true amounts of fatty acids in connection with the method for quantitative evaluation of paper chromatograms developed by Kaufmann et al. It is shown that residual mobile phase influences the conversion of fatty acid to copper soap in a manner which forwards the linearity of the photometric response curves. When scanning narrow cross sections of the spots true linearity following Beer''s law exists up to spot contents of about 20 [mu]g.This publication has 1 reference indexed in Scilit:
- Direct Quantitation of Paper Chromatograms of Radioactivity from 14C Labelled Fatty Acids.Acta Chemica Scandinavica, 1961