Restriction fragment length polymorphisms of horse class II MHC genes observed using various human alpha‐and beta‐chain cDNA probes

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Abstract
Summary. Genomic DNA isolated from 20 horses was digested with up to six restriction endonucleases and subjected to southern blot hybridization analysis using various human class II alpha‐ and beta‐chain cDNA probes. A high degree of restriction fragment length polymorphism (RFLP) was found for the DQα, DPß, DQß and DRß probes, about 20 polymorphic bands being detected for each. DRα showed 2–4 polymorphic bands, whereas no evidence for DPα‐like genes was found. A number of correlations of RFLPs with individual alloantisera were apparent.