Transforming growth factors from human colonic carcinoma cells induced molecular alterations in untransformed mouse AKR embryo fibroblasts
- 1 November 1986
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 10 (11) , 891-895
- https://doi.org/10.1016/0309-1651(86)90107-4
Abstract
Over 700 polypeptide spots could be detected by two-dimensional electrophoretic analyses of membranes prepared from the murine AKR fibroblastic cells. Out of this abundance of polypeptides, only 9 polypeptide spots were found to be differentially expressed between the untransformed AKR-2B cells and their methylcholanthrene-transformed counterparts, the AKR-MCA cells. Treatment of the untransformed AKR-2B cells with transforming growth factors, prepared from the serum-free conditioned medium of HCT 116 MOSER human colonic carcinoma cells, induced the altered expression of 6 of these polypeptides which paralleled the electrophoretic profile of their permanently transformed counterparts, the AKR-MCA cells.This publication has 6 references indexed in Scilit:
- CELL-SURFACE FEATURES ASSOCIATED WITH DIFFERENTIATION-INDUCTION OF METHYLCHOLANTHRENE-TRANSFORMED AKR-2B FIBROBLASTS BY N,N-DIMETHYLFORMAMIDE1985
- SELECTIVE PHOSPHORYLATION OF CYTOSOL PROTEINS ASSOCIATED WITH TRANSFORMATION AND RESTORATION OF NORMAL PHENOTYPE IN AKR MOUSE EMBRYO FIBROBLASTS1985
- Induction of plasma membrane alterations in AKR-2B mouse embryo fibroblasts by endogenous growth factors from malignant human cellsInternational Journal of Cancer, 1984
- RESTORATION OF NORMAL GROWTH-CONTROL AND MEMBRANE ANTIGEN COMPOSITION IN MALIGNANT-CELLS BY N,N-DIMETHYLFORMAMIDE1984
- COMPARISON OF INTRACELLULAR AND EXTRACELLULAR TRANSFORMING GROWTH-FACTORS FROM NONTRANSFORMED AND CHEMICALLY TRANSFORMED MOUSE EMBRYO CELLS1983
- TRANSFORMING GROWTH-FACTOR PRODUCTION BY CHEMICALLY TRANSFORMED-CELLS1981