HIGH-LEVEL IODINATION OF MONOCLONAL-ANTIBODY FRAGMENTS FOR RADIOTHERAPY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 25  (3) , 367-370
Abstract
Two different murine monoclonal antibody Fab fragments specific for p97, a melanoma-associated antigen, were labeled with I-131 at high activity levels without excessive chemical damage. Up to 20 mg of Fab were labeled with up to 300 mCi of I-131 using the chloramine-T method and large working volumes at room temperature. As much as 90% of the initial activity was recovered as labeled product. The labeled Fab varied in their sensitivity to radioiodination damage, as measured by an in vitro cell-binding assay. Radioiodination was performed safely using a remote iodination apparatus. The final product was of radiopharmaceutical quality suitable for clinical diagnosis and experimental radiotherapy [of metastatic melanoma] in humans.

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