N‐Fluoroacetyl‐d‐glucosamine as a Molecular Probe of Lysozyme Structure
Open Access
- 1 November 1971
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 23 (2) , 343-348
- https://doi.org/10.1111/j.1432-1033.1971.tb01627.x
Abstract
The synthesis and nuclear‐magnetic‐resonance spectra of N‐fluoroacetyl‐d‐glucosamine are described, together with 19F chemical shifts for some monosaccharides substituted with fluorine at different positions. The sugar derivative N‐fluoroacetyl‐d‐glucosamine is inhibitory towards lysozyme, 50% of full inhibition being given by about 15 mM. At pH 5.4, addition of this sugar derivative to lysozyme solutions results in a concentration‐dependent broadening and down‐field shift in the 19F‐resonance, of both anomeric forms. The α‐anomeric form is shifted more than the β‐form.Keywords
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