Pulmonary surfactant‐associated polypeptide SP‐C in lipid micelles: CD studies of intact SP‐C and NMR secondary structure determination of depalmitoyl‐SP‐C(1–17)
Open Access
- 10 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (3) , 261-265
- https://doi.org/10.1016/0014-5793(95)00216-v
Abstract
The surfactant‐associated polypeptide C (SP‐C) is a 35‐residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP‐C in an organic solvent showed that SP‐C could readily insert into fluid 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine bilayers. The present paper describes further physical‐chemical studies of intact SP‐C and its N‐terminal 17‐residue polypeptide fragment, depalmitoyl‐SPC(1–17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP‐C in an ordered lipid environment, and thus present new insights for rational design of SP‐C analogs for therapeutic purposes.Keywords
This publication has 43 references indexed in Scilit:
- Determination of scalar coupling constants by inverse Fourier transformation of in-phase multipletsJournal of Magnetic Resonance (1969), 1992
- Spatial structure of (34–65)bacterioopsin polypeptide in SDS micelles determined from nuclear magnetic resonance dataJournal of Biomolecular NMR, 1992
- Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteinsJournal of Magnetic Resonance (1969), 1989
- Improved solvent suppression in one- and two-dimensional NMR spectra by convolution of time-domain dataJournal of Magnetic Resonance (1969), 1989
- Combined use of hard and soft pulses for ω1 decoupling in two-dimensional NMR spectroscopyJournal of Magnetic Resonance (1969), 1988
- Nuclear magnetic resonance investigation of the conformation of δ-haemolysin bound to dodecylphosphocholine micellesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Conformation of glucagon in a lipid-water interphase by 1H nuclear magnetic resonanceJournal of Molecular Biology, 1983
- A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromoleculesBiochemical and Biophysical Research Communications, 1980
- Digital filtering with a sinusoidal window function: An alternative technique for resolution enhancement in FT NMRJournal of Magnetic Resonance (1969), 1976