Enzymatic Formation of Dicarbonyl Sugars: C-2 Oxidation of 1↠6 Disaccharides Gentiobiose, Isomaltose and Melibiose By Pyranose 2-Oxidase from Trametes Multicolor

Abstract
Pyranose 2-oxidase, a homotetrameric FAD-flavoprotein from the fungus Trametes multicolor, catalyzes the oxidation of 1→6 disaccharides gentiobiose (β-D-Glcp-(1→6)-D-Glc), melibiose (α-D-Galp-(1→6)-D-Glc), and isomaltose (α-D-Glcp-(1→6)-D-Glc) at C-2 of their reducing moiety. The products were identified as their hydrazone derivatives.

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