Gel Entrapment of Enzyme

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Abstract
The feasibility of gel entrapment of enzymes as a strategy for enzyme correction was tested using polyacrylamide gel containing the antitumor enzyme, asparaginase. Intravenous and/or intraperitoneal injections of labeled enzyme particles into rats resulted in a significant though transient reduction in serum asparagine levels. Cultivated human fibroblasts grown in the presence of particles failed to incorporate significant amounts of labeled gel or enzyme. In contrast, when particles were given intravenously, intracellular localization in the reticuloendothelial system could be demonstrated. These studies raise serious questions as to the potential usefulness of this approach for treatment of inborn errors of metabolism.

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