Continuous Cultivation of Glucose-Limited Bean Cells (Phaseolus vulgarisL.) in a Modified Bacterial Fermentor
- 1 December 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 30 (6) , 1223-1231
- https://doi.org/10.1093/jxb/30.6.1223
Abstract
A commercially available bacterial fermentor was modified to allow the continuous cultivation of glucose-limited bean cells (Phaseolus vulgaris L.) at a defined pH. A steady state was reached using a dilution rate of 0·004 h−1 and was maintained for 4 months; during this period the dry weight and packed cell volume of the culture remained constant; the yield for glucose was 0·34 (dry wt./dry wt) and for carbon 0·27 (g atom/g atom). Glucose-limited cells had lower C/N ratios both for cytoplasm and cell walls than cells in the exponential phase of growth in batch cultures.This publication has 8 references indexed in Scilit:
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