Identification and quantification of 2-amino-3,4,8-trimethylimidazo-[4,5-f]quinoxaline (4,8-DiMeIQx) in beef extract

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Abstract
2-Amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-Di-MeIQx) was found in bacteriological-grade beef extract by liquid chromatographies with electrochemical detection and a photodiode array detector. 4,8-DiMeIQx was estimated to be present at a level of 10.0 ng per g of beef extract, and to account for 9% of the total mutagenicity of the extract.