Phased A‐tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature
- 27 November 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 509 (2) , 235-238
- https://doi.org/10.1016/s0014-5793(01)03148-9
Abstract
Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A‐tracts in a low temperature‐dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A‐tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A‐tracts through its C‐terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A‐tracts.Keywords
This publication has 20 references indexed in Scilit:
- The pathway for perception and transduction of low-temperature signals in SynechocystisThe EMBO Journal, 2000
- Promoter upstream bent DNA activates the transcription of the Clostridium perfringens phospholipase C gene in a low temperature-dependent mannerThe EMBO Journal, 1999
- Probing the physical basis for trp repressor-operator recognitionJournal of Molecular Biology, 1999
- Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase α subunitProceedings of the National Academy of Sciences, 1998
- H‐NS: a modulator of environmentally regulated gene expressionMolecular Microbiology, 1997
- An upstream activating sequence containing curved DNA involved in activation of the Clostridium perfringens plc promoterMicrobiology, 1996
- Fluorescence polarization — a new tool for cell and molecular biologyNature, 1995
- Promoter architecture in the flagellar regulon of Bacillus subtilis: high-level expression of flagellin by the sigma D RNA polymerase requires an upstream promoter element.Proceedings of the National Academy of Sciences, 1995
- A Third Recognition Element in Bacterial Promoters: DNA Binding by the α Subunit of RNA PolymeraseScience, 1993
- Application of fluorescence energy transfer and polarization to monitor Escherichia coli cAMP receptor protein and lac promoter interaction.Proceedings of the National Academy of Sciences, 1990