Transformation of Bovine Cells in vitro by Polyoma Virus, and the Properties of the Transformed Cells

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Abstract
Polyoma virus was capable of transforming cell cultures of bovine embryonic lung. The transformation was characterized by an abnormal morphology and growth pattern; there were rapidly growing cells having stellate or triangular shape and lying at random, criss-crossing one another. The transformed cells did not contain infectious polyoma virus. A "tumor" antigen was demonstrated in the cells by complement fixation tests using serum from hamsters bearing polyoma-induced tumors. The viral susceptibility of the polyoma-transformed cells was tested and compared with that of SV40 [Simian virus 40]-transformed and normal bovine cells. The polyoma-transformed cells were capable of propagating foot-and-mouth disease virus to fairly high titers whereas in the other cells low titers were obtained. The SV40-transformed cells showed a decreased susceptibility to bovine enterovirus and pseudorabies virus in comparison with the polyoma-transformed and normal cells.