RECEPTORS FOR INSULIN-LIKE PEPTIDES (ILAs) IN RAT RETICULOCYTES AND ERYTHROCYTES.
- 1 March 1981
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 108 (3) , 1087-1090
- https://doi.org/10.1210/endo-108-3-1087
Abstract
Rat reticulocytes possess receptors for an insulin-like somatomedin peptide ILAs. At 20 C, pH 7.4 and 1O8 cells per ml, the binding of 125I-ILAs reached a steady-state at 120 min. Binding was partially reversible at 20 C and totally reversible at 37 C. Insulin did not compete with ILAs at concentrations as high as 10 μg/ml. Insulin-like growth factor II (IGF II) competed with the same potency as ILAs itself, while insulin-like growth factor I (IGF I) was five times less potent. The optimum pH for 125I-ILAs binding was 8.5. Mature erythrocytes possessed seven times less binding sites than reticulocytes. These data suggest that the somatomedins might mediate the effect of growth hormone on erythropoiesis.Keywords
This publication has 4 references indexed in Scilit:
- Insulin Binding to Erythrocytes: A Negative Correlation with Red Cell Age*Journal of Clinical Endocrinology & Metabolism, 1980
- Partial Purification, Characterization, and Assay of a Slightly Acidic Insulin-Like Peptide (ILAs) from Human Plasma*Journal of Clinical Endocrinology & Metabolism, 1978
- SERUM SOMATOMEDIN ACTIVITY MEASURED AS SULPHATION FACTOR IN PERIPHERAL, HEPATIC AND RENAL VEINS OF PATIENTS WITH ALCOHOLIC CIRRHOSISActa Endocrinologica, 1978
- Independent variation of β-adrenergic receptor binding and catecholamine-stimulated adenylate cyclase activity in rat erythrocytesLife Sciences, 1976