Mesoblastic tumours following intraperitoneal injections of 1 : 2 : 5 : 6-Dibenzanthracene in a fatty medium

Abstract
Attention was first drawn to 1 : 2 : 5 : 6-dibenzanthracene as a carcinogenic substance during an inquiry into the fluorescent spectra of coal tars carried out by W. Y. Mayneord in collaboration with E. L. Kennaway at this Institute in 1927 and later continued by I. Hieger (1930). Since then the substance has been prepared in a very pure form by J. W. Cook (1931, a), who has made some closely allied compounds which are also carcinogenic (1931, b, c; 1932). Kennaway (1930) and Hieger (1930) have already recorded the causation of epitheliomata by 1:2:5: 6-dibenzanthracene dissolved in benzene applied to the skin of mice ; and Burrows, Hieger and Kennaway (1932) have described the production of connective tissue tumours in rats and mice by injecting 1:2:5: 6-dibenzanthracene dissolved in lard into the subcutaneous tissues. These tumours conformed to the usually accepted criteria of malignancy; grafted strains have now reached the 23rd and 36th generation in mice and the 18th and 20th generation in rats. Technique . The present paper sets out some results of the intraperitoneal injection of fatty solutions of 1 : 2 : 5 : 6-dibenzanthracene. This substance was dissolved in a concentration of 0 • 4 per cent, in olive oil by heating at temperatures not exceeding 100° and the solution was then emulsified in 3 volumes of 5 per cent, gum acacia in water usually made alkaline to 8·2 in order to assist emulsification. The concentration of the hydrocarbon in the emulsion was thus 0·1 per cent.*

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