The Lipopolysaccharide from Pseudomonas aeruginosa NCTC 8505

Publisher Website
Google Scholar
Abstract
Structural studies were carried out on the O-specific fraction from the lipopolysaccharide of P. aeruginosa NCTC 8505, Habs serotype 03. The O-specific polysaccharide has a tetrasaccharide repeating-unit containing residues of L-rhamnose (Rha), 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamido-2-deoxy-L-galacturonic acid (GalNAcA) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (BacNAc2). The following structure was assigned to the repeating-unit: .fwdarw. 3)Rhap(.beta.1 .fwdarw. 6)GlcpNAc(.alpha.1 .fwdarw. 4)GalpNAcA(.alpha.1 .fwdarw. 3)BacpNAc2(.alpha.1 .fwdarw.. The parent lipopolysaccharide is a mixture of S, R and SR species and its high P content is partly due to the presence of triphosphate residues, as found for other lipopolysaccharides from P. aeruginosa. In addition to P, heptose, a 3-deoxyoctulosonic acid and amide-bound alanine, the core oligosaccharide contains glucose, rhamnose and galactosamine (molar proportions 3:1:1). The rhamnose and part of the glucose are present as unsubstituted pyranoside residues; other glucose residues are 6-substituted.