Loss of HLA‐C locus specificities from stored lymphocytes1 and de novo synthesis following incubation at 37°C

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Abstract
The variable expression of the C locus specificities at the cell surface is a major problem in HLA typing and the phenomenon was clearly documented by the International Cell Exchange Program. In contrast with HLA-A and -B, the C locus specificities readily lost from cells following storage at room temperature for a few days or in overnight incubation at 37.degree. C in the presence of protein synthesis inhibitors. These specificities can be made to reappear by making the cells metabolize at 37.degree. C for 8-20 h in a nutrient medium. Of the positive C locus reactions lost after 7-day storage at room temperature, 69% were recovered after overnight incubation at 37.degree. C. The reappearance was dependent on protein synthesis since the presence of cycloheximide during incubation completely blocked the recovery. A simple overnight incubation is very useful in enhancing C locus expression on older cells.

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