Pregenomic RNA Is Required for Efficient Incorporation of Pol Polyprotein into Foamy Virus Capsids
Open Access
- 1 October 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (19) , 10069-10073
- https://doi.org/10.1128/jvi.76.19.10069-10073.2002
Abstract
The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly.Keywords
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