Effect of Freezing of Human Fecal Specimens upon the Isolation of Nuclear Dehydrogenating Clostridia

Abstract
Fresh and frozen human fecal specimens were used for the isolation of nuclear dehydrogenating clostridia (NDC). The total bacterial count and the number of lecithinase-positive bacteria were lower in frozen samples than in fresh samples, but the frequency of isolation of NDC from frozen specimens was higher than from fresh specimens. [5.beta.-androsteu-3,17-dione was used as substrate].

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