Monoclonal Antibodies Recognizing Structural Components of Murine Retroviruses Including an FMR Antigen on Protein p12

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Abstract
Monoclonal antibodies were prepared from mice and rats immunized with Friend leukemia virus and BALB/c xenotropic virus. By immunoprecipitation of 125I-labeled and [35S]methionine-labeled viruses and by protein blotting, 10 antibodies were found to react with the viral components p12, p15, p30, gp70 and p15E/p12E. A dot-immunobinding assay was found to be a reliable method to type the antibody reactivity with different murine leukemia viruses (MuLV). When tested on a panel of ecotropic and xenotropic MuLV, the antibodies revealed the following antigenic specificities: ecotrop-specific on p15E/p12E; xenotrop-specific on p15E; group-specific on p30 and p15E; Friend-Moloney-specific on gp70; Friend-Rauscher-specific on gp70 and p15. Of particular interest is a cytotoxic antibody recognizing a Friend-Moloney-Rauscher determination localized on p12.

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