ANALYSIS OF TRANSFER-RNA POPULATION OF MOUSE MAMMARY-GLANDS INFECTED WITH A LATENT MAMMARY-TUMOR VIRUS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (9) , 3082-3085
Abstract
Mammary gland transfer RNA''s (tRNA''s) of C3H mice infected with mammary tumor virus were analyzed in the preneoplastic state and compared to tRNA''s of virus-free C3Hf mice and another uninfected strain, C57BL/6, which is completely resistant to cancer. This quantitative study was based on the ability of each tRNA to fix its corresponding amino acid. The amount of each of the 17 tRNA''s tested was identical for the 3 mammary glands. tRNA populations during lactation correlated with the amino acids incorporated into the lactoproteins synthesized, which indicates adaptation of the tRNA''s to protein biosynthesis. Qualitative chromatographic studies on reverse phase capillary columns type 5 of 10 aminoacyl-tRNA''s did not reveal any difference in the isoacceptor elution profiles. This shows that no new isoaccepting tRNA is associated with the mammary tumor virus at that stage, and no viral modification of host tRNA occurred.

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