The PR264/c-myb connection: expression of a splicing factor modulated by a nuclear protooncogene.
- 15 December 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (24) , 11683-11687
- https://doi.org/10.1073/pnas.89.24.11683
Abstract
We have previously reported that expression of the c-myb gene in normal avian thymic cells proceeds through the intermolecular recombination of ET (thymus-specific) and c-myb coding sequences, thereby generating a novel type of c-myb product. Antisense transcripts expressed from the ET locus encode the extremely well-conserved splicing factor PR264/SC35. We now show that the human PR264 promoter sequences contain several myb-recognition elements that efficiently interact in vitro with the c-myb DNA-binding domain. Moreover, expression from the PR264 promoter is transactivated, both in vitro and in cultured cells, by different c-myb products. Thus, the PR264 gene is most likely a physiological target for the c-myb family of transcription factors.Keywords
This publication has 24 references indexed in Scilit:
- A potential splicing factor is encoded by the opposite strand of the trans-spliced c-myb exon.Proceedings of the National Academy of Sciences, 1992
- Specific DNA Binding by c-Myb: Evidence for a Double Helix-Turn-Helix-Related MotifScience, 1991
- Mechanisms of Alternative Pre-mRNA SplicingScience, 1991
- The myb oncogeneBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1990
- The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 geneCell, 1989
- Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-mybCell, 1989
- Viral myb oncogene encodes a sequence-specific DNA-binding activityNature, 1988
- Nucleotide sequence of chicken c-myb complementary DNA and implications for myb oncogene activationNature, 1986
- Analysis of the expression of the 4F2 surface antigen in normal and neoplastic fibroblastic human cells of embryonic and adult originExperimental Cell Research, 1985
- Differential expression of the amv gene in human hematopoietic cells.Proceedings of the National Academy of Sciences, 1982