Identification of 5,8-oxyretinoic acid isolated from small intestine of vitamin A-deficient rats dosed with retinoic acid.

Abstract
A retinoid was isolated by a multistep procedure from the small intestines of vitamin A-deficient rats given a single dose of retinoic acid. The compound, designated 8II, was pure, as demonstrated by 4 high-pressure liquid chromatographic procedures. It was positively identified as 5,8-oxyretinoic acid by UV spectrophotometry, mass spectrometry and spectral and chromatographic comparison to known compounds. It is probable that 5,8-oxyretinoic acid was produced from 5,6-epoxyretinoic acid under the acidic conditions used in the isolation. It is highly probable that the natural product is 5,6-epoxyretinoic acid.

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