Measurement of acetylcholine receptor function in microcircuit-coupled myoblasts
- 1 January 1993
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Biomedical Engineering
- Vol. 40 (11) , 1122-1126
- https://doi.org/10.1109/10.245629
Abstract
An electrophysiological measurement principle for long-term, noninvasive monitoring of the nicotinic acetylcholine receptor (nAChR) function is described. The measurement is based on the ability to record agonist-induced depolarizations of clonal myoblasts that have formed high impedance seals with extracellular microcircuit electrodes. The technique appears promising for several types of assays and environmental monitoring applications.Keywords
This publication has 16 references indexed in Scilit:
- Surface-machined micromechanical membrane pumpPublished by Institute of Electrical and Electronics Engineers (IEEE) ,2002
- Microfabrication Techniques for Integrated Sensors and MicrosystemsScience, 1991
- A Neuron-Silicon Junction: A Retzius Cell of the Leech on an Insulated-Gate Field-Effect TransistorScience, 1991
- New class of microminiature Joule — Thomson refrigerator and vacuum packageCryogenics, 1990
- Frequency domain analysis of membrane capacitance of cultured cells (HeLa and myeloma) using the micropipette techniqueBiophysical Journal, 1990
- Direct measurement of the concentration- and time-dependent open probability of the nicotinic acetylcholine receptor channelBiophysical Journal, 1990
- Sealing cultured invertebrate neurons to embedded dish electrodes facilitates long-term stimulation and recordingJournal of Neuroscience Methods, 1989
- Reconstitution of purified acetylcholine receptors with functional ion channels in planar lipid bilayers.Proceedings of the National Academy of Sciences, 1980
- Synapse Formation Between Two Clonal Cell LinesScience, 1977
- Properties of a clonal muscle cell line from rat heartExperimental Cell Research, 1976