Adsorption of 14-Labeled Gramicidin S on Cell of Bacteria
- 1 October 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 90 (4) , 1087-1091
- https://doi.org/10.1093/oxfordjournals.jbchem.a133560
Abstract
Gramicidin S (GS) containing 14C-labelled proline was synthesized by a solid-phase method, and the labeled GS dihydrochloride was obtained as crystals. The labeled GS exhibited same antibacterial activity as natural GS. Strains sensitive to GS (B. subtilis and S. aureus) and an insensitive strain (E. coli) were treated with the labeled GS, and the amount of the labeled GS adsorbed on the cells was measured. GS was adsorbed rapidly on the cells of the sensitive strains; the amount adsorbed increased linearly with GS concentration up to 1–1.5μg/ml, and at a lower rate at above 1.5μ/ml. GS molecules covered most of the cell surface at the minimum inhibitory concentration of 1.5μ/ml, the number of molecules adsorbed per cell was 1.3–1.4×105. No GS was adsorbed by the insensitive strain.Keywords
This publication has 1 reference indexed in Scilit:
- The antibacterial action of some basic amino acid copolymersArchives of Biochemistry and Biophysics, 1956