Root, Root Hair, and Symbiotic Mutants of the Model Legume Lotus japonicus
Open Access
- 1 January 2002
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 15 (1) , 17-26
- https://doi.org/10.1094/mpmi.2002.15.1.17
Abstract
To gain an overview of plant factors controlling nodule number and organogenesis, an extensive screening using model legume Lotus japonicus was carried out. This screening involved 40,000 M2 seeds, and 32 stable mutant lines were isolated. From these, 16 mutant lines maintaining the phenotypic variation were selected and genetically analyzed. With respect to nodule number, four loci were identified, Ljsym77, Ljsym78, slippery root (slp), and radial organization1 (rdo1). The former two mutants have an increased number of nodules, while the latter two have a decreased number. Ljsym78-1 and Ljsym78-2 are hypernodulating mutants with a branched root system and were found to be allelic to Ljsym16. The phenotype of the Ljsym77 mutant was highly pleiotropic, being deficient in light and gravity responses. The slp mutant was isolated as a low-nodulating mutant lacking root hairs. Concerning nodule organogenesis, nine symbiotic loci were identified, including the two loci alb1 and fen1. Mutants affecting the developmental process of nodule organogenesis were placed in three phenotypic categories: Nod¯ (Ljsym70 to Ljsym73), Hist¯ (alb1-1, alb1-2, and Ljsym79), and Fix¯ (fen1, Ljsym75, and Ljsym81).Keywords
This publication has 55 references indexed in Scilit:
- Providing the basis for genomics in Lotus japonicus: the accessions Miyakojima and Gifu are appropriate crossing partners for genetic analysesMolecular Genetics and Genomics, 2001
- Responses of a Model Legume Lotus japonicus to Lipochitin Oligosaccharide Nodulation Factors Purified from Mesorhizobium loti JRL501Molecular Plant-Microbe Interactions®, 2001
- A Bacterial Artificial Chromosome Library of Lotus japonicus Constructed in an Agrobacterium tumefaciens-Transformable VectorMolecular Plant-Microbe Interactions®, 2001
- Analysis of ENOD40 expression in alb1, a symbiotic mutant of Lotus japonicus that forms empty nodules with incompletely developed nodule vascular bundlesMolecular Genetics and Genomics, 2000
- Mtsym6, a Gene ConditioningSinorhizobium Strain-Specific Nitrogen Fixation inMedicago truncatulaPlant Physiology, 2000
- T-DNA Tagging of Nodulation- and Root-Related Genes in Lotus japonicus: Expression Patterns and Potential for Promoter Trapping and Insertional MutagenesisMolecular Plant-Microbe Interactions®, 1999
- Nodule Organogenesis and Symbiotic Mutants of the Model Legume Lotus japonicusMolecular Plant-Microbe Interactions®, 1998
- Mutagenesis of faba bean (Vicia faba L.) and the identification of five different genes controlling no nodulation, ineffective nodulation or supernodulationEuphytica, 1995
- A Mutation inVicia fabaResults in Ineffective Nodules with Impaired Bacteroid Differentiation and Reduced Synthesis of Late NodulinsJournal of Experimental Botany, 1992
- A non-nodulating alfalfa mutant displays neither root hair curling nor early cell division in response to Rhizobium meliloti.Plant Cell, 1989