Aggregation of Oral Streptococci Selected by Growth on Human Saliva from Different Glands

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Abstract
From a plaque sample of one volunteer five streptococcal strains were selected by batch-wise enrichment cultures on clarified human whole (CHW), parotid (PAR) and extraparotid, mainly submandibular-sublingual (SMSL) saliva. Binding of salivary components was tested by aggregation studies with the same types of saliva. The SMSL selected strains S.sanguis NY 584 and S.oralis NY 585 and NY 586 aggregated with CHW and SMSL saliva but not with PAR saliva, suggesting mucous components were involved with aggregation. S.oralis NY 582, the PAR selected strain, was not aggregated by CHW and SMSL saliva indicating no interaction with mucous substances. PAR saliva also did not induce aggregation of NY 582, suggesting binding of PAR components to this strain did not result in the formation of aggregates. The CHW selected strain, S.oralis NY 583, showed autoaggregation. S.sanguis NY 584 grew well on PAR saliva and CHW-mediated aggregation was inhibited by PAR saliva, implying an interaction with PAR components. S.oralis NY 586 did not grow well on PAR saliva and CHW-mediated aggregation of this strain was not inhibited by PAR saliva, implying no interaction with PAR saliva. These results suggest that binding of salivary components to these bacteria is a prerequisite for the nutritional use of these salivary components.