Enhanced UV-Detection of Barbiturates in HPLC Analysis by on-Line Photochemical Reaction
- 1 July 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (11) , 2207-2216
- https://doi.org/10.1080/01483919008049024
Abstract
After irradiation with UV-light a significant change in the UV-spectra of a number of barbiturates has been observed. The photochemical reaction results mainly in a bathochromic shift and appearance of a strong spectral band at 270nm, due to a dealkylation reaction in position 5 of the barbiturate molecule. By using an on-line photoreactor in HPLC analysis, barbiturates can be analyzed with much higher specificity in biological samples and, using absorbance ratios, identification of these drugs in unknown samples is facilitated.This publication has 4 references indexed in Scilit:
- Simultaneous determination of thiopental and its metabolite, pentobarbital, in blood by high-performance liquid chromatography and post-column photochemical reactionJournal of Pharmaceutical and Biomedical Analysis, 1989
- Enhanced ultraviolet detection in high-performance liquid chromatographic analysis of drugs by “on-line” photochemical reactionJournal of Chromatography A, 1989
- Photolytic Derivatization for Improved LCEC Determinations of Pharmaceuticals in Biological FluidsJournal of Chromatographic Science, 1985
- Improved detectability of barbiturates in high performance liquid chromatography by post-column ionizationAnalytical Chemistry, 1978