Methyl-3 hydroxy-6 quinazoline dione-2-4, a new compound for labelling plant cells. Application to the study of two inhibitors of cytokinesis.

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Abstract
The action of MHQD [methyl-3 hydroxy-6 quinazoline dione-2-4] on a meristematic root cell population of Allium sativum L. was studied to investigate the possibility of using MHQD as a morphological labeler and to specify the advantages and disadvantages of the method. After a brief treatment, MHQD causes the immediate appearance of binucleate cells. These cells have normal kinetic parameters and there is no significant loss of synchronism between their 2 nuclei. This sample of labeled cell population goes synchronously throughout the cell cycle. Unlike caffeine, MHQD does not modify the mitosis duration nor the DNA synthesis ratio. MHQD seems suitable as a morphological labeler, useful for the study of the action of various cell cycle inhibitors. Two examples of application of this method are given.