INSULIN AND CYCLIC AMP RELEASE BY ISOLATED-INCUBATED ISLETS OF LANGERHANS

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Abstract
The release of both insulin and cyclic[c]AMP by isolated islets was measured in the presence of various glucose concentrations in mice. Under the experimental conditions used a glucose-dependent insulin and cAMP release was observed. In both cases, this increase showed a direct dependence on glucose concentration. When the magnitude of the increases induced by glucose were compared, a breakdown in correlation was seen. While in the presence of 3 mg glucose/ml both the hormone and nucleotide showed almost an identical percentage rise (to 613 .+-. 50 (SEM) .mu.u[microunits]ml/h and 6.79 .+-. 0.49 pg/O.2 ml per h, respectively), at 0.6 mg glucose/ml the proportion increase in cAMP release was far above that of insulin. The lack of a strict parallelism between insulin and cAMP release described here would suggest that the cAMP mechanism, though important, is not the only mechanism involved in glucose-induced insulin release.

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