Ile‐Lys‐Val‐Ala‐Val (IKVAV)‐containing laminin α1 chain peptides form amyloid‐like fibrils
- 27 September 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 530 (1-3) , 48-52
- https://doi.org/10.1016/s0014-5793(02)03393-8
Abstract
The Ile‐Lys‐Val‐Ala‐Val (IKVAV) sequence derived from laminin‐1 promotes cell adhesion, neurite outgrowth, and tumor growth and metastasis. Here, we examined amyloid formation of an IKVAV‐containing peptide (LAM‐L: AASIKVAVSADR, mouse laminin α1 chain 2097–2108). The LAM‐L peptide was stained with Congo red and exhibited fibrils in electron microscopy with a characteristic cross‐β X‐ray diffraction pattern. Further, infrared spectra of LAM‐L suggested a β‐sheet structure. These results indicate that LAM‐L forms amyloid‐like fibrils. We also examined amyloid‐like fibril formation of LAM‐L analogs. The neurite outgrowth activity of the LAM‐L analogs was closely related to their amyloid‐like fibril formation.Keywords
This publication has 43 references indexed in Scilit:
- Identification of a novel human islet amyloid polypeptide β-sheet domain and factors influencing fibrillogenesisJournal of Molecular Biology, 2001
- Design of a Peptide Undergoing α-β Structural Transition and Amyloid Fibrillogenesis by the Introduction of a Hydrophobic DefectChemistry – A European Journal, 1998
- Cell Binding Sequences in Mouse Laminin α1 ChainJournal of Biological Chemistry, 1998
- Characterization of High Affinity Binding between Laminin and the Acute-phase Protein, Serum Amyloid APublished by Elsevier ,1997
- Laminin Interactions Important for Basement Membrane Assembly Are Promoted by Zinc and Implicate Laminin Zinc Finger-like SequencesJournal of Biological Chemistry, 1996
- Identification of Cell Binding Sites in the Laminin α1 Chain Carboxyl-terminal Globular Domain by Systematic Screening of Synthetic PeptidesJournal of Biological Chemistry, 1995
- Structure‐activity study of a laminin α1 chain active peptide segment Ile‐Lys‐Val‐Ala‐Val (IKVAV)FEBS Letters, 1995
- Role of the SIKVAV Site of Laminin in Promotion of Angiogenesis and Tumor Growth: An In Vivo Matrigel ModelJNCI Journal of the National Cancer Institute, 1992
- Laminin and its neurite outgrowth‐promoting domain in the brain in Alzheimer's disease and Down's syndrome patientsJournal of Neuroscience Research, 1992
- THE RELATION OF THE PROPERTIES OF CONGO RED-STAINED AMYLOID FIBRILS TO THE β-CONFORMATIONJournal of Histochemistry & Cytochemistry, 1972