Abstract
The effect of the conventional mesophilic anaerobic one-step digestion, and of two-phase processes with and without pre-pasteurisation and pasteurisation of the digested sludge on three non-enveloped viruses was investigated. Reovirus Type 1 and bovine Enterovirus (ECBO-LCR-4) were inactivated completely by the one-step mesophilic anaerobic digestion process with a mean hydraulic retention time (MHRT) of 20 days, as well as by the two-phase process with a MHRT of two days in the anaerobic pretreatment at 33°C or 20°C and eight days in the main digestion at 33°C, with and without prepasteurisation. None of the processes inactivated bovine Parvovirus (strain Haden) completely. Even by pasteurisation of the digested sludge this virus could not be inactivated in all cases.

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