Cell Lineage Analysis by Intracellular Injection of Fluorescent Tracers
- 26 September 1980
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 209 (4464) , 1538-1541
- https://doi.org/10.1126/science.6159680
Abstract
Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.This publication has 5 references indexed in Scilit:
- Embryonic development of the Glossiphoniid leech Theromyzon rude: Characterization of developmental stagesDevelopmental Biology, 1980
- Embryonic cell lineages in the nervous system of the Glossiphoniid leech Helobdella triserialisDevelopmental Biology, 1980
- Cell Lineage Analysis by Intracellular Injection of a Tracer EnzymeScience, 1978
- Killing of single neurones by intracellular injection of proteolytic enzymesNature, 1977
- Size Limit of Molecules Permeating the Junctional Membrane ChannelsScience, 1977