Mycelial proteins from 7 isolates of E. gyrosa and 5 isolates of E. parasitica were extracted from acetone powders, separated on polyacrylamide gels by disc electrophoresis and stained for general proteins, esterase activity or .beta.-glucosidase activity. By simple visual inspection, the 12 uncoded general protein gels were separated into the 2 spp. The enzyme gels of both species were more easily differentiated. Very little or no .beta.-D-glucosidase and general esterase activities were noted on E. parasitica gels. Although the extract sample load (.apprx. 220 .mu.g protein) was standardized and qualitative and quantitative intraspecific variations were noted, these did not interfere with species differentiation. Protein and enzyme profiles from a 30-yr-old isolate of E. gyrosa compared favorably with new isolates. These data corroborate the separation of the species on the basis of morphology.