A double staining technique for improved contrast of thin sections from Spurr-embedded tissue

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Abstract
A double staining procedure using potassium permanganate and lead citrate is described. The technique, involving treatment of sections with a 1% solution of potassium permanganate followed by lead citrate staining, is shown to be more effective in producing contrast in Spurr-embedded tissue than the conventional uranyl acetate – lead citrate method.

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