Thin‐layer electrophoretic separation of monosaccharides, oligosaccharides and related compounds on reverse phase silica gel

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Abstract
The electrophoretic mobilities of monosaccharides, oligosaccharides, sugar alcoho and sugar acids were determined in 0.3 M borate buffer, pH 10, using thin-layer electrophoresis on silanized silica gel, pretreated with octanol-1. A rapid separation of number of sugars, occurring in foods, could be achieved. Using a 0.05–0.1 M neutra solution of barium acetate as electrolyte, thin-layer electrophoresis allowed exceller and rapid separation as well as identification of all common uronic acids which are constituents of many acidic polysaccharides.