INTRACELLULAR DISTRIBUTION AND SOME PROPERTIES OF HEXOKINASE OF BOVINE ADRENAL MEDULLA

Publisher Website
Google Scholar
Abstract
The intracellular distribution of the hexokinase of the adrenal medulla was studied. Most of the hexokinase was found, in about equal amounts, in the mitochondria and the nonparticulate cytoplasm. The amount of hexokinase associated with the mitochondria was shown to be dependent on the hexokinase concentration in the medium surrounding the mitochondria.The hexokinase of the adrenal medulla was shown to be noncompetitively inhibited by adenosine diphosphate (ADP) with respect to glucose and adenosine triphosphate (ATP). It was competitively inhibited with respect to glucose by mannose, fructose, and galactose. Glucose-6-phosphate (0.09 M) did not inhibit the hexokinase. The data on product inhibition and initial velocity analysis support the concept of an ordered sequential mechanism for adrenal-medulla hexokinase.

This publication has 2 references indexed in Scilit: