CA++-INDUCED CATARACT

  • 1 January 1982
    • journal article
    • research article
    • Vol. 22  (2) , 263-267
Abstract
Cataracts in cultured rabbit lenses were produced by elevation of internal Ca. Experimental procedures were successful in increasing levels of total and bound Ca2+, often without significant changes in Na, K or water content. Although the excess in Ca was predominantly associated with water-soluble proteins and was freely diffusible, a significant amount was bound to membranes and cytosol water-insoluble proteins. In lenses with a 10-fold increase in total Ca2+, the bound Ca2+ increased 2-fold, nearly 35% of which remained fixed to water-insoluble and membrane proteins after exhaustive (72 h) dialysis. Over 95% of the Ca2+ in water-soluble protein fractions was removed by dialysis.

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