Isolation and nucleotide sequence of theThiobacillus ferrooxidansgenes for the small and large subunits of ribulose 1,5‐bisphosphate car☐ylase/oxygenase
- 4 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 292 (1-2) , 85-89
- https://doi.org/10.1016/0014-5793(91)80840-y
Abstract
The genes encoding for the large (rbcL) and small (rbcS) subunits of ribulose‐1,5‐bisphosphate car☐ylase (RuBisCO) were cloned from the obligate autotrophThiobacillus ferrooxidans, a bacterium involved in the bioleaching of minerals. Nucleotide sequence analysis of the cloned DNA showed that the two coding regions are separated by a 30‐bp intergenic region, the smallest described for the RuBisCO genes. TherbcLandrbcSgenes encode polypeptides of 473 and 118 amino acids, respectively. Comparison of the nucleotide and amino acid sequences with those of the genes forrbcLandrbcSfound in other species demonstrated that theT. ferrooxidansgenes have the closest degree of identity with those ofChromatium vinosumand ofAlvinoconcha hessleriendosymbiont. BothT. ferrooxidansenzyme subunits contain all the conserved amino acids that are known to participate in the catalytic process or in holoenzyme assembly.Keywords
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