1H NMR Thermodynamical Analysis of the Interactions of Proflavine with Self-Complementary Deoxytetranucleotides of Different Base Sequence

Abstract
Enthalpies and entropies of complex formation (1:1, 2:1, 1:2, and 2:2 complexes) between proflavine and tetranucleotides of different base sequence have been determined by 500 MHz proton NMR chemical shifts, enabling the contributions to be differentiated for the formation of different types of complexes in solution. Comparison of the calculated thermodynamical parameters has lead to an understanding of the nature of the intermolecular interactions responsible for the formation of dye complexes with the different tetranucleotides.