Comparison of Plasmids Isolated from Romet-30-ResistantEdwardsiella ictaluriand Tribrissen-ResistantEscherichia coli
- 1 March 1993
- journal article
- Published by Oxford University Press (OUP) in Journal of Aquatic Animal Health
- Vol. 5 (1) , 9-15
- https://doi.org/10.1577/1548-8667(1993)005<0009:copifr>2.3.co;2
Abstract
Edwardsiella ictaluri, the etiological agent of enteric septicemia of channel catfish (ESC) is the leading cause of bacterial disease in commercially raised channel catfish Ictalurus punctatus. The only drug approved by U.S. Food and Drug Administration for use against ESC is Romet-30. Recently, several isolates were obtained that had a naturally occurring resistance to Romet-30. On further characterization these isolates were shown to possess a 55-kilobase (kb) plasmid that encodes resistance to the drug. We compared Romet-30-resistant E. ictaluri and a Tribrissen-resistant strain of Escherichia coli (strain 1898) isolated from equine cystitis. Antimicrobial profiles, plasmid screening, restriction digest, and Southern blot analysis indicated that the two plasmids are very similar. The resistance afforded to the E. coli was encoded by a 55-kb plasmid. Each of the R plasmids conferred resistance to Romet-30, tetracycline, and Terramycin (oxytetracycline). The R plasmid from E. coli strain 1898 wa...Keywords
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