Gold coating of non‐conductive membranes before matrix‐assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect
- 31 January 2005
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 19 (5) , 605-610
- https://doi.org/10.1002/rcm.1831
Abstract
Acquisition of tandem mass spectra from peptides or other analytes deposited on non‐conductive membranes is inhibited on instruments combining matrix‐assisted laser desorption/ionization with tandem time‐of‐flight analyzers (MALDI‐TOF/TOF™) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI‐TOF/TOF™ systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
This publication has 23 references indexed in Scilit:
- Photoionization Pathways and Free Electrons in UV-MALDIAnalytical Chemistry, 2004
- Surface Adsorption and Transfer of Organomercaptans to Colloidal Gold and Direct Identification by Matrix Assisted Laser Desorption/Ionization Mass SpectrometryJournal of the American Chemical Society, 2004
- Integrating Histology and Imaging Mass SpectrometryAnalytical Chemistry, 2004
- Laser desorption/ionization cluster studies for calibration in mass spectrometryThe European Physical Journal D, 2003
- Time-of-flight mass spectrometry of bioorganic molecules by laser ablation of silver thin film substrates and particlesJournal of Mass Spectrometry, 1998
- Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mappingElectrophoresis, 1997
- Mass Spectrometry of Proteins Directly from Polyacrylamide GelsAnalytical Chemistry, 1996
- Matrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresisAnalytical Chemistry, 1994
- Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometryAnalytica Chimica Acta, 1990
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970