Measurement of Relative Quantum Yields of Strongly Absorbing Solutions
- 1 November 1970
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 24 (6) , 588-590
- https://doi.org/10.1366/000370270774372344
Abstract
Formulas are derived which enable one to measure relative fluorescence quantum yields of strongly absorbing solutions. Relative fluorescence yield data are presented for quinine in N/10 H2SO4 at four different concentrations, demonstrating that the formulas are correct to within ±3% for sample absorbances ranging from 1.36 to 0.017. The heart of these formulas, an expression giving the fraction of incident light absorbed behind the entrance slit of the emission monochromator, is presented in graphical form.Keywords
This publication has 4 references indexed in Scilit:
- FLUORESCENCE OF 5‐METHYLCYTOSINEPhotochemistry and Photobiology, 1970
- Practical aspects of the calibration and use of the Aminco-Bowman spectrophotofluorometerAnalytical Biochemistry, 1967
- Fluorescence assay of high concentrations of DPNH and TPNH in a spectrophotofluorometerAnalytical Biochemistry, 1965
- Some experiments with spectrofluorimeters and filter fluorimetersThe Analyst, 1957