SPECIFICITY OF BLEOMYCIN-INDUCED CYTO-TOXIC EFFECTS ON ATAXIA TELANGIECTASIA LYMPHOID-CELL LINES

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (5) , 1817-1823
Abstract
Bleomycin exposure evoked a specific sensitivity in 5 ataxia telangiectasia (AT) long-term lymphoblastoid cell lines [human] when compared to lines derived from 4 normal individuals or 3 patients with xeroderma pigmentosum. At all concentrations tested or after each treatment regimen, statistically significant differences in viability and cytogenetic damage were obvious, with the AT cell lines demonstrating reduced survival and increased chromosomal breakage. Similar differences were not observed following treatment of all cell lines with mitomycin C. The normal and xeroderma pigmentosum cells appear capable of overcoming the effects of bleomycin during a 48- or 72-h recovery period while the AT cell lines could not. This specific response to bleomycin constitutes the 1st demonstration of increased chromsome breakage in vitro in long-term AT lymphoblastoid cell lines.