METABOLISM OF CARBOHYDRATES BY PSEUDOMONAS SACCHAROPHILA III

Open Access
Publisher Website
Google Scholar
Abstract
The ability of P. saccharophila to oxidize D-arabinose requires selection of mutant strains capable of adapting to this substrate. The sugar is oxidized to D-arabono- [gamma]- lactone by a specific DPN-linked dehydrogenase. The lactone is hydrolyzed enzymically to D-arabonic acid, which is further dehydrated by D-arabonic dehydrase to 2-keto-3-deoxy-D-arabonic acid. 2-Keto-3-deoxy-D-arabonic acid is dehydrogenated with DPN and cleaved to yield pyruvic and glycolic acids. The carboxyl group of pyruvic acid is derived from C1 of arabinose, while the carbinol group of glycolic acid arises from C5.