Rearing Douglas-Fir Tussock Moth Larvae on Synthetic Media for the Production of Nuclear-Polyhedrosis Virus1

Abstract
A technique for rearing larvae of the Douglas-fir tussock moth, Hemerocampa pseudotsugata McDunnough, in large numbers on a synthetic medium for the production of nuclear polyhedrons virus is described. The insect was reared from the egg stage generally to the 4th and 5th instar before infection. The larvae appeared normal in every way, reaching an average weight of 310 mg. Mortality during the larval stage prior to infection was very low.

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